The evolutionary and functional paradox of cerato-platanins in fungi
Abstract : Cerato-platanins (CPs) form a family of fungal small-secreted cysteine-rich proteins (SSCPs) and are of particular int
Source : AEM
Auth : Renwei Gao, Mingyue Ding, Siqi Jiang, Zheng Zhao, Komal Chenthamara, Qirong Shen, Feng Cai, and Irina S. Druzhinina
keywords : Evolution, fungal-bacterial interactions, fungal-fungal interactions, gene duplication, lateral gene transfer, natural selection, plant immune response, protein secretion, rhizosphere colonization, small secreted cysteine-rich proteins

Cerato-platanins (CPs) form a family of fungal small-secreted cysteine-rich proteins (SSCPs) and are of particular interest not only because of their surface activity but also their abundant secretion by fungi. We performed an evolutionary analysis for 283 CPs from 157 fungal genomes with the focus on the opportunistic plant-beneficial and mycoparasitic fungus Trichoderma. Our results revealed the long evolutionary history of CPs in Dikarya fungi that have undergone several events of lateral gene transfer and gene duplication. Three genes were maintained in the core genome of Trichoderma, while some species have up to four CP-encoding genes. All Trichoderma CPs evolve under stabilizing natural selection pressure. The functional analysis of CPs in T. guizhouense and T. harzianum revealed that only EPL1 is active at all stages of the development but plays a minor role in interactions with other fungi and bacteria. The deletion of this gene results in increased colonization of tomato roots by Trichoderma spp. Similarly, the biochemical tests of the heterologously produced EPL1 by Pichia pastoris support the above claims. Based on the obtained results, we conclude that the function of CPs is probably linked to their surfactant properties and the ability to modify the hyphosphere of submerged mycelium and thus facilitate the nutritional versatility of fungi. The effector-like properties do not sufficiently describe the diversity and evolution of these proteins in fungi as they are also maintained, duplicated, or laterally transferred in the genomes of non-herbivore fungi.


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